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KMID : 0368419970400020110
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Journal of Plant Biology
1997 Volume.40 No. 2 p.110 ~ p.114
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Transcription of Human Interleukin-2 Gene in Nicotiana tabacum Driven by caMV35S Promoter
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Park Soo-Min
Hong Choo-Bong
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Abstract
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Human interleukin-2 (IL-2) gene was inserted next of the 35S-transcript promoter of cauliflower mosaic virus in a binary vector and introduced into tobacco genome by Agrobacterium-mediated transformation. Genomic DNA blot analysis of the in vitro regenerated transgenic tobacco confirmed that the introduced human IL-2 gene was successfully integrated into tobacco genome. RNA blot analyses were carried out for the IL-2 transcript to examine expression of the introduced IL-2 gene. Specific signals only from the transgenic tobacco plants indicated that the introduced human IL-2 gene was properly transcribed. The transgenic tobacco plants were self-pollinated and the seeds were harvested. Differences in the level of the transcript were more apparent during the germination of the seeds and between the T_2 generation tobacco plants.
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